Which molecule is used to cut out a section of DNA? A. Restriction endonuclease. B. Reverse transcriptase C. Gel electrophoresis D. DNA polymerase​

Answers

Answer 1
Answer: A, restriction endonuclease

Explanation: None just know it

Related Questions

In which ways might ocean currents be like streams and rivers on land?

Answers

What are the chances you doing this week and

Pour each bag of candy into a separate container or dish so you can see all the colors in each bag. Assign a letter to each color of chocolate candies and fruit candies, and record your key.
For example, red=R, purple=P


Without looking, randomly choose nine candies from each container one at a time. Using the letter symbols, record each candy as it is removed. Record your results using the following table. Example, R for red, Bl for blue, and so on.




Color

Color

Color

Color

Color

Color

Color

Color

Color
Number of different colors in total for this trial
Fruit Candy Trial 1




















Fruit Candy Trial 2




















Fruit Candy Trial 3




















Chocolate Candy Trial 1




















Chocolate Candy Trial 2




















Chocolate Candy Trial 3






















Count the number of species in each sample. Each color represents a different plant in the habitat.

Example: R, Bl, G, G, Y, Y, G, Bl, Bl has 4 species of plants (R, Bl, G, and Y).
You will then be dividing the # of different species (which is the # of different colors) in the next step.

Calculate the diversity index of both the bag of chocolate candies and fruit candies using the following formula for each trial.


Diversity Index = # of species
# of samples

Example:
R, Bl, G, G, Y, Y, G, Bl, Bl is 4/9 = 0.44
The # of species is 4 because there are 4 different colors.

Put the diversity index for each of the 6 trials in this table. This will always be a number less than 1, but more than zero.
Calculate the average from the three trials.
Diversity Index Table

Sample
Trial 1
Trial 2
Trial 3
Average
Fruit








Chocolate










Analysis (Make sure you scroll down to see all 7 questions.)
How did the diversity index values vary from the chocolate candies and fruit candies? Which habitat is the most diverse?


Why are communities that are more diverse usually more stable? Include resistance to disease and predation in your answer.


Assume two habitats have the same number of species. One habitat is predominantly one species with just a few of the other species. The other habitat has equal numbers of all the different species. Which will have the highest diversity index?

Why do you think the process was repeated three times?


There are many human-caused losses of biodiversity, such as habitat destruction and introduction of invasive species. Are there any natural events that could alter the diversity index?


How do invasive species change the diversity index?


What do you think would happen to a habitat if the plant diversity declines?


please help fast!!!!
thank you

Answers

Invasive species can potentially change the diversity index in a number of ways. They may come to an area where they have no natural predators, leading to overpopulation and rapid consumption of ecosystem resources.

Invasive species are capable of causing extinctions of native plants and animals, reducing biodiversity, competing with native organisms for limited resources, and altering habitats.

The impacts of alien invasive species at the ecosystem level include changes to trophic structures, changes in the availability of resources such as water and nutrients, and changes in the disturbance regimes.

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Part of the cell cycle is shown in this simplified model. This part of the cell cycle is best described as the process that-

Part of the cell cycle is shown in this simplified model. This part of the cell cycle is best described

Answers

Answer:a

Explanation:

Answer:

It's A

Explanation:

The simplest type of a substance is a compound true or false

Answers

Answer:

True

Explanation:

Elements and compounds are examples of pure substances. The simplest units of elements and compounds are atoms and molecules. We find that these pure substances only contain one type of particle.

3419059779 ppppppp1234​

Answers

Answer:uh- Thank u for the free points kind sir or ma'ma also can u plz give me Brainlist have an good day! <3

Explanation:

what makes zebra mussels an invasive species?

Answers

Answer:

Zebra mussels negatively impact ecosystems in many ways. They filter out algae that native species need for food and they attach to--and incapacitate--native mussels. Power plants must also spend millions of dollars removing zebra mussels from clogged water intakes.

If a newborn baby weighs 8 pounds and 4 ounces how much does it weigh in grams

Answers

3856 :)

Have a good day

Answer:

3810.18 value 454

Explanation:

if the baby is 8 pounds and 4 oz

Which component of the blood is part of the body’s immune system?

Answers

Answer:

White Blood Cells

Explanation:

6. Surface currents in the ocean can influence
land areas they flow past because they
a. earthquakes on, stimulate tectonic plates
b. streams on, carry extra water onto the land
c. climates of, carry warmer or colder water great distances
d. rock formations on, prevent erosion

Answers

Answer:

c. climates of, carry warmer or colder water great distances.

Explanation:

Surface currents in the ocean can affect the temperature of the surrounding air and land areas they flow past, influencing regional climates. Warm ocean currents can bring warmer and more humid weather to the adjacent land, while cold ocean currents can have the opposite effect, cooling the air and land temperatures. This can have significant effects on local ecosystems, agriculture, and economies.

What causes the characteristic thrashing motion of nematodes?

A. the fluid-filled body cavity
B. the pseudocoelom that acts as a hydroskeleton
C. the blunted head and pointed tail​

Answers

B. the pseudocoelom that acts as a hydro skeleton causes the characteristic thrashing motion of nematodes.

The longitudinal muscles of nematodes are contracted to move them. The animal moves by thrashing back and forth because to the tremendous internal pressure that forces the body to flex rather than flatten. Nematodes only have longitudinal muscles, not the two bands of muscles found in other worms. They can only move by clenching the lengthy muscles on either side of their body and wiggling forward, which explains their distinctive thrashing motion.The pseudocoelom serves as a lubricant between tissues, houses coelomocytes, and generates turgor-hydrostatic pressure for the entire animal.Because it is fluid-filled, the fluid's hydrostatic qualities enable a more rigid construction, greatly improving the efficiency of the skeletal system and movement.

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Answer: B. the pseudocoelom that acts as a hydroskeleton

Explanation:

QUICK I NEED HELP ASAP

QUICK I NEED HELP ASAP

Answers

Answer:

Cell,tissue,organ,organ system

Explanation:

I already took this test

how pigments absorb and reflect wavelengths of light. Light energy:

the properties of light. Know the colors of light in the visible spectrum. Which has most energy? The least energy?​

Answers

Answer:

jhrgthfvdsvfhwegf

Explanation:

jhdstefuysdzvdnfgjewhfghkvdakjad

LIMITED TIME, HELP URGENTLY !!!!!!!!!!!!!
The climate shifts in an ecosystem, causing the rainy seasons to
become shorter and water to become more scarce overall. What is
the most likely outcome of this environmental change?
Select one:

O No change in the equilibrium of each population

O All populations will increase in size.

O Some populations will expand while others decline.

O All populations will decline in size.

Answers

Answer:

Some populations will expand while others decline.

Explanation:

The populations in the environment will probably be impacted as the climate changes, the rainy seasons shorten, and water becomes more scarce. While some that are less suited may have a lesser probability of surviving, the organisms that are more acclimated to the new conditions will have a higher likelihood of survival and reproduction.

The size of the populations in the ecosystem will alter as a result of this shift in the environment's impact on population dynamics. The size of certain populations may expand because they are more suited to the new circumstances, while the size of other populations may decrease because they are less adaptable. Given that the environment would change as a result of this scenario, it is important to note that no change in the equilibrium of each population is not likely to occur.

Which situation does NOT describe a function of the reproductive system?

ensures species survival

regulates the menstrual cycle

allows sperm and eggs to combine

Answers

Answer:

regulates the menstrual cycle

Explanation:

The other 2 answers are apart of the reproductive system

4. Is surface water considered to be ground water? Why, or why not?

Answers

Answer:

Surface water is a key component to the hydrologic cycle. Surface water is any body of water above ground, including streams, rivers, lakes, wetlands, reservoirs, and creeks. ... Water that seeps deep into the ground is called groundwater. Surface water and groundwater are reservoirs that can feed into each other.

A substance requires energy to move across a membrane when it moves from:

A. Both
B. High Voncentration to low concentration
C. Low concentration to High concentration​

Answers

A substance requires energy to move across a membrane when it moves from (C) Low concentration to High concentration

The process of moving a substance across the cell membrane from high to low concentration is known as passive transport, which does not require energy. Active transport, on the other hand, is the process of moving a substance from low to high concentration, which requires energy.

The movement of molecules against their concentration gradient (low to high concentration) requires an energy source, which is usually in the form of ATP (adenosine triphosphate). Low concentration to High concentration. During active transport, the substance moves against its concentration gradient, which means it moves from an area of lower concentration to an area of higher concentration.

This process requires energy, which is provided by ATP. Active transport is responsible for the uptake of nutrients, such as glucose and amino acids, in cells. The process of active transport is crucial in maintaining the proper concentration of ions in cells. Additionally, active transport is essential for maintaining the pH of cells and the function of enzymes. Overall, active transport is an energy-intensive process that is vital for cellular homeostasis and function. The correct answer is C.

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Major horticulture grouping

Answers

Floriculture, olericulture, pomology and post-harvest technology are major horticulture grouping

Floriculture is a branch of ornamental horticulture concerned with growing and marketing flowers. olericulture a branch of horticulture that deals with the production, storage, processing, and marketing of vegetables.

Pomology is the study and cultivation of fruits it is also known as fruticulture. Pomology is emerging field in science and technology for production and handling of temperate fruit and nut crop. Post harvest technology is responsible for  protection, conservation, processing, packaging, distribution, marketing of fruits and vegetables .

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Why is so important for dentists to check what kind of heart disease the patients have before treatment, and why some patients who have heart disease require to take pre-medication before some dental treatments. If a 10 years old kid who has heart murmur, should he undergo general anesthesia for dental treatment due to he has excessive caries.

Answers

because it can cause stress which can hurt the heart

Which immune process is most similar to a child being exposed to pathogens by eating dirt? immunization injections cilia filtration natural killer cells anti-histamine reactions

Answers

The process most similar to a child eating dirt and being exposed to pathogens is called immune priming. Immune priming is when a person is exposed to a weak antigen or pathogen, stimulating a response from their immune system that will help them better respond to a secondary, stronger exposure to the pathogen.

What is priming?

Antigen-specific T helper cell precursors initially come into touch with an antigen during priming. The ensuing interaction between T helper cells and B cells, which triggers the production of antibodies, is crucial. [1] When antigen is delivered to naive lymphocytes in an immunogenic state, priming of those cells takes place (capable of inducing an immune response). The primed cells will then develop into memory cells or effector cells, respectively, that can mount a stronger and quicker response to subsequent and forthcoming immunological threats. [2]

Dendritic cell antigen presentation is necessary for priming naive T cell activation. Naive CD8 T cells that have been primed produce cytotoxic T cells that can kill pathogen-infected cells directly. According on the sort of signals they receive, CD4 cells can grow into a wide range of effector cell types.

What is the function of CD4 cells?

Co-receptors of TCRs (T cell receptors), CD4 cells help TCRs communicate with antigen-presenting cells. The antigen-presenting MHS class II molecules are specifically targeted by the TCR complex and CD4 cells. These CD4 cells' extracellular D1 domain interacts with the MHC Class II 2 region to form a bond. Because of this interaction, the tyrosine kinase Lck is able to phosphorylate ITAMs (immunoreceptor tyrosine activation motifs), which are attached to the cytoplasmic tail of CD4 on the cytoplasmic domains of CD3. The T cell receptors' ability to produce messages is enhanced by this.

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I am struggling answering this question:Discuss the structural differences between polysaccharides starch, cellulose, and glycogen, explaining how the differences in the structure contribute to the functional property of the molecule.

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Starch: Starch is the main storage carbohydrate source in plants.

Starch serves as a carbohydrate energy store

Cellulose: Cellulose is the main structural component of the cell wall of plants.

Cellulose is involved in the building of cellular structures like cell walls.

Glycogen: Glycogen is the main storage carbohydrate energy source of fungi and animals.

Glycogen serves as a carbohydrate energy store.

Starch, cellulose, and glycogen are polysaccharides found in organisms. Starch is found in plants as their major storage form of carbohydrates. Linear chains of starch are called amylose and when branched they are called amylopectin. Glycogen is similar to amylopectin but is highly branched. It is the major carbohydrate storage form in animals and fungi. Cellulose is a linear polysaccharide, which forms hydrogen bonds among several cellulose chains to form a fibrous structure. It is the major component of the cell wall of plants, some algae, and fungi. Thus, the main difference between starch cellulose and glycogen is their role in each organism.

HELP ASAP THIS IS MAJOR GRADE

HELP ASAP THIS IS MAJOR GRADE

Answers

Answer:ITS B GRAVITY PULLS AN INWARD FORCE!

Answer:

Answer A

Explanation:

Gravity keeps things on the ground meaning the gravitational force is going down.

Using the brake pedal analogy, explain the impact on the cell cycle of one mutated tumorsuppressor gene allele versus two mutated tumor suppressor alleles.If there is only one mutated tumor allele, the effect is the same as if there are no mutated genes, pressing thebrakes on the car only when prompted. However two mutated tumor suppressor alleles presents a differentoutcome as it appears as if the brakes are indefinitely pushed downwards, preventing any future cell cycleprogression.

Answers

Tumor suppressor genes work by preventing cancer cell division and thus preventing tumor formation. Because mutations in this gene result in loss of function, it is usually recessive.

This means that tumor suppressor genes slow the growth of cancer cells. It inhibits tumor growth. The car or cancer formation can be halted even if one of the brakes (one tumor suppressor gene) fails. However, because tumor suppressor gene mutations are recessive, if two brakes fail, the car will not stop. Furthermore, the tumor will be expressed and formed.

If only one mutated tumor allele exists, the effect is the same as if no mutated genes exist, with the brakes applied only when necessary.

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Hlo! Guys help me and. if you answer see what offer I am going to give you​

Hlo! Guys help me and. if you answer see what offer I am going to give you

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Answer:

1:Ctrl+C.cut: Ctrl+x. paste : Ctrl+v

2: Ctrl+S

3: Ctrl+O

4:Ctrl+W

5:shift+ctrl+left

shift+Ctrl+right

shift+ctrl+down

shift+ctrl+up

6: Ctrl +U

7: Ctrl+B

8:Ctrl+i

9:Ctrl+R

10:Ctrl+E

11:Ctrl+R

12:Ctrl +J

As a result of your Zombie Apocalypse, the grass at a local park will no longer be mowed. What type of succession is this (primary or secondary)? Describe the stages of succession that will occur in the next 100 years.
What type of succession is this depicting?

As a result of your Zombie Apocalypse, the grass at a local park will no longer be mowed. What type of

Answers

Secondary succession is the kind of succession that might develop in a neighborhood park after the Zombie Apocalypse if the grass wasn't cut anymore and pioneer species would first colonize the bare soil.

Thus, pioneer species like weeds and grasses would colonize the bare soil and disturbed regions in the initial stage of secondary succession. These species can swiftly adapt to a new habitat since they are frequently resilient and rapidly developing.

In secondary succession, the pioneer species are the early settlers multiply and spread, the soil conditions become more stable and draw in additional species like shrubs and tiny trees. These animals are modifying the environment in a way that makes it possible for bigger and more diversified populations of creatures to live in more complicated settings.

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the area of the brain required to understand language is

Answers

Answer:

Wernicke's region is a basic language region in the back better fleeting flap interfaces than Broca's region by means of a brain process. Wernicke's region is basically engaged with the perception. By and large, this region has been related with language handling, whether it is composed or spoken.

ASAP PLS!!! LOTS OF POINTS
Compare the two graphs.
1. What conclusion can you draw about the fur color of wolves that live in these two
very different habitats? Use evidence from the graphs to support your answer. 4
points
2. What advantage might the difference in fur color have? 2 points

ASAP PLS!!! LOTS OF POINTSCompare the two graphs.1. What conclusion can you draw about the fur color

Answers

Answer:

Based on the graph, we can draw the following conclusions:

The graph compares the fur color of wolves that live in a forested habitat (black bars) and those that live in a tundra habitat (gray bars). The forested habitat wolves have mainly gray and black fur, while the tundra habitat wolves have mainly white fur.

This suggests that wolves that live in different habitats have adapted to blend in with their surroundings to help them hunt or avoid predators. The wolves in the forested habitat are better camouflaged with darker fur, while those in the tundra habitat are better camouflaged with lighter fur.

The difference in fur color provides an advantage for wolves because it helps them survive in their respective environments. Wolves with better camouflage are less likely to be seen by their prey or predators, making it easier for them to hunt or avoid danger.

In summary, the graph shows that wolves that live in different habitats have adapted to their surroundings by changing their fur color, which provides them with an advantage for survival in their environments.

Explanation:

In the process of
Choose...
, DNA is used as a template for making another type of nucleic acid called
Choose...
. The process begins when the enzyme
Choose...
binds to a region called the
Choose...
.

Answers

In the process of transcription, DNA is used as a template for making another type of nucleic acid called RNA. The process begins when the enzyme RNA polymerase that binds to a region called the promoter sequence.

What is the cell process of genetic transcription?

The cell process of gene transcription is a phenomenon for which a nucleic acid called RNA is created by means of one of a single-strand sequence of DNA, which is used as a template to generate a complementary RNA sequence that binds by complementarity pair basis rules. During transcription, an enzyme called RNA polymerase progressively adds nucleotides to the growing RNA chain, in a process that requires the binding with a DNA sequence called the promoter.

Therefore, with this data, we can see that the cell process of genetic transcription is based on the use of an RNA polymerase that initially binds to a DNA template strand.

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sea otters are examples of a species

Answers

Answer: yes

Explanation:they are keystone species

When a DNA molecule replicates, a. The hydrogen bonds along the sugar-phosphate chain are broken. b. The covalent bonds along the sugar-phosphate chain are broken. c. The hydrogen bonds between the nitrogenous base pairs are broken. d. The covalent bonds between the nitrogenous base pairs are broken.

Answers

Answer:

A

Explanation:

Answer:

C

Explanation:

The DNA chain breaks the hydrogen bonds between nitrogenous bases when it replicates so that the bases are exposed to DNA polymerase. Breaking the backbone would ruin the molecule.

Why and how do living things do cellular respiration?

Answers

Answer:

Cellular respiration is a metabolic pathway that converts nutrients into energy (ATP) that organisms need to live. ... The oxygen you breathe is used to break down the nutrients you consume through food in order to make ATP, while carbon dioxide and water are generated as waste

Explanation:

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Answer: All living things use cellular respiration to turn organic molecules into energy. Cellular respiration is the chemical process of breaking down food molecules in order to create energy in the form of adenosine triphosphate (ATP). ... Cellular respiration usually occurs in the presence of oxygen.

Other Questions
A student designs a gravity experiment. She will time how long it takes different objects to fall from her school's roof to the ground. She will use threeobjects: a 2-foot-long board, a toy car, and a paper bag. What is wrong with her experiment's design? (1 point)O The objects do not have the same size, shape, or mass, so any differences in drop times cannot be attributed to one variableO The experiment does not test enough objectsO Gravity will not affect how long it takes for the objects to fall to the ground.O The school roof is not tall enough to test the effect of gravity on different objects. What are the interest cost and the total amount due on a six-month loan of $1,500 at 13. 2 percent simple annual interest?. Jack was out at a restaurant for dinner when the bill came. His dinner came to $14. He wanted to leave a 31% tip. How much was his meal plus the tip, before tax, in dollars and cents? A new homeowner needs to determine the length of his rectangular backyard before he goes to the store forfencing. He knows that the area of the yard is 51 square meters and that the width is 5. 2 meters longerthan the length. Which equation could be solved to determine the dimensions of the backyard, where the lengthis / and the width is w?0=w2+5. 2w -51o0=12+5. 21-514w + 10. 4 = 5141 +10. 4 = 51 18. Electronegativity is the total negative charge of the electrons in an atom. T. O True F. O False The ages of pennies at a particular bank follow a nearly normal distribution with mean 10.44 years with standard deviation 9.2 years. Say you take random samples of 30 pennies, find the mean age in each sample, and plot the distribution of these means. Which of the following are the best estimates for the center and spread of this distribution? O mean = 10.44, standard error = 9.2 O mean = 10.44, standard error 9.2/30 = 0.31 O mean = 10.44/30 = 0.348, standard error (9.2/30)2 = 0.094 O mean = 10.44, standard error = 9.2/V30 = 1.68 TRUE/FALSE. judaism is a wing of judaism that counteracted reform judaism, modifying jewish tradition in a limited manner. Convert 99% to a decimal Lewis Co. reports the following results for May. Prepare a flexible budget report showing variances between budgeted and actual results. Budgeted Actual Sales 950 per unit $1,470,000Variable expenses 380 per unit 588,000Fixed expenses (total) $144,500 135000Units produced and sold 1,530 1,330 List variable and fixed expenses separately. after the completion of the sale, for entrepreneurs, the relationship with the client does not matter. (T/F) Which of the following ISACA certifications, while not specifically a security certification, contains many information security systems auditing components and is only offered a few times per year?a. CISAb. CISMc. CGEITd. CRISC Find afor each arithmetic series. Sa=240 and d=-2 Is y=3/4x proportional? what does the pilot think grownups are most interested in Write down the value of the digit 5 in 75389 write your answer in words. the nurse manager used a mediator to help resolve conflicts on the unit. during the mediation process, the nurse manager saw signs of potential team building. one key concept of an effective team is: Peter shoveled a driveway today to earn some extra money. He spent $4.10 on ashovel and $5.60 on lunch. He charged $18.50 to shovel the driveway.What was his profit for shoveling the driveway?A. $8.80B. $20.00C. $28.20D. $17.00 a compound composed of 3.3% h, 19.3% c, and 77.4% o has a molar mass of approximately 60 g/mol. what is the molecular formula of the compound? molecular formula: hco A change in quantity suppliedis caused only by:A change in quantity supplied is caused only by: taxes O price O capital O technology Need help Completing the spreadsheet to determine the economically optimal investment in additional security.Deliverable:Spreadsheet that uses formulas to answer the assignment question:1. Populate the cells in columns C-F using formulas2. Write a formula in cell B23 that returns the amount of the optimal additional investment in security.HINT: One way to do this is to use a VLOOKUP function in cell B23.Homework 4 Example CalculationsFor Homework 4, several calculations are required to find the optimal investment. For our purposes,we will be using the investment amount of $250,000 for all our example calculations.1. Expected LossThe formula for Expected Loss consists of the following:The probability a breach will occur x The estimated loss associated with a breach.Using the homework example, if our company invests an amount of $250,000 into oursecurities, the probability of breach decreases from a 40% chance to a 30% chance.However, the total amount of money at risk stays constant at $10,000,000. Therefore, if wemultiply our new probability of breach, .3, by our total potential loss, $10,000,000, we getan expected loss of $3,000,000.2. Marginal InvestmentThe formula for Marginal Investment consists of the following:Current Investment Amount Previous Investment AmountMarginal Investment is the extra amount of money our company is investing into a businessarea (in this case securities). For our example, if we are considering investing an amount of$250,000, our marginal investment would be our current investment amount, $250,000,minus our previous investment amount, $0. This would give us a marginal investment equalto $250,000. In addition, if you instead considered investing $500,000, the marginalinvestment would be our current investment, $500,000, minus our previous investment,$250,000, giving us a marginal investment once again equal to $250,000.3. Marginal BenefitThe formula for Marginal Benefit consists of the following:The Previous Expected Loss The Current Expected LossThe Marginal Benefit refers to the total amount an additional investment reduces theExpected Loss. In simpler terms, if our company invests more money, we expect that theexpected loss will decrease. For example, if we are investing $250,000, our current expectedloss is $3,000,000. Meanwhile, the previous expected loss is $4,000,000 for investing $0. Ifwe take $4,000,000 and subtract $3,000,000, we get a marginal benefit of $1,000,000. Thismeans that by investing an additional $250,000 into securities, we were able to reduce ourexpected loss by $1,000,000.4. Net BenefitThe formula for Net Benefit consists of the following:Marginal Benefit Marginal InvestmentWhen our company invests more and more into securities, we want to make sure that we aregetting a positive return on our investment. To see if our investments are giving us positiveresults, we calculate the net benefit on each investment. Net Benefit will compare theadditional amount of money we have invested into securities to the amount that our expectedloss decreased. The goal should be that the expected loss decreases by more than the amountof money we invest. For example, if our investment of $250,000, we have a marginalinvestment of $250,000 and a marginal Benefit of $1,000,000. This would mean that ourtotal net benefit will equal $1,000,000 - $250,000, or $750,000. (*Note: Net Benefits can benegative if the expected loss decrease is lower than the additional amount invested).5. Cumulative Net BenefitThe formula for Cumulative Net Benefit consists of the following:Current Net Benefit + All Previous Net BenefitsTo find the optimal investment, we need to find the point at which investing more moneywill not produce positive results. To find this point, we calculate the cumulative Net Benefit.For our investment of $250,000, this would equal the current net benefit of $750,000 plus allother previous net benefits (since we only have one prior investment, we would use that netbenefit of $0.) This would equal $750,000 + $0 or $750,000. For a further demonstration,lets say our investment of $500,000 gives us a net benefit of $250,000. If we were tocalculate its cumulative net benefit, it would be $250,000 + ($750,000 + $0) = $1,000,000.